Regulatory

Part:BBa_J100071

Designed by: Ben Clarkson   Group: Campbell M Lab   (2012-07-05)

cadA promoter

The promoter for the cadBA operon in E. coli.

Induced by low extracellular pH ([http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE-IN-MAP&object=EG10131 cadA gene]).

CadC senses external pH, by low pH preventing the formation of a disulfide bond, activating CadC. When activated, CadC binds to two sites upstream of the cadBA promoter, Cad1 and Cad2. The binding of CadC to these sites activates transcription by releasing the H-NS molecules that are bound to, and repressing, the cadBA operon ([http://ecocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=PD00436 CadC function in cadBA operon]).

Cloned into pSB1A8.

Similar to K112405.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None